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. Author manuscript; available in PMC: 2021 Mar 15.
Published in final edited form as: Cancer Res. 2020 Jul 22;80(18):3880–3891. doi: 10.1158/0008-5472.CAN-20-1049

Figure 1.

Figure 1.

CHD4 interacts with HIF-1 and HIF-2. (A-D) Co-IP with IgG, anti-HIF-1α (A), anti-HIF-2α (B), anti-HIF-1β (C), or anti-CHD4 (D) antibody in MDA-MB-231 cells exposed to 1% O2 for 6 hrs. WCL, whole cell lysate. (E and F) Purified GST, GST-HIF-1α domains (E), or GST-HIF-2α domains (F) were incubated in the presence of glutathione Sepharose beads with cell lysates from HEK293T cells transfected with FLAG-CHD4, followed by immunoblot assay with anti-CHD4 antibody or Ponceau S staining.