Table 1.

Tsf1 residues predicted to be involved in iron and anion binding

	Amino-lobe						Carboxyl-lobe
	Iron				Anion		Iron				Anion
	Da	Y	Y	H	T	R	D	Y	Y	H	T	R
	63b	95	188	249	120	124	392	426	517	585	452	456
Blattodea
(5c)	+d	+	+	Q	+	+	+	+	+	+	+	+
Coleoptera
(3)	+	+	+	Q	+	+	+	+	+	+	+	+
(2)	+	+	+	Q	+	+
Diptera
(21)	+	+	+	Q	+	+
(13)	E	+	+	S	+	+
(12)	E	+	+	T	+	+
(1)	+	+	+	P	+	+
(1)	E	+	+	M	+	+
Hemiptera
(5)	+	+	+	Q	+	+
(4)	E	+	+	P	+	+
Hymenoptera
(16)	+	+	+	Q	+	+
(1)	+	+	+	Q	+	L
Lepidoptera
(13)	+	+	+	Q	+	+
Orthoptera
(1)	+	+	+	Q	+	+	+	+	+	+	+	+

^aConsensus iron and anion binding residues of serum transferrin, lactoferrin and ovotransferrin sequences are shown.

^bPosition numbers are based on human serum transferrin.

^cThe number in parentheses is the number of species within an order to have the particular sequence shown. Species and accession numbers are listed in Table S1.

^dPlus signs indicate conservation of consensus residues; empty spaces indicate a lack of conservation.