Fig. 2.

Knockdown of Xist increased inflammatory response in female mouse macrophage J774A.1 cells upon LPS stimulation. a Relative expression of Xist in J774A.1 cells 24 h after siRNA (siXist or control siRNA 30 pM) transfection. b Fold change in the expression of Il6 mRNA in J774A.1 cells in response to LPS stimulation (1 µg/ml for 4 h) relative to control 24 h after transfection with siRNA targeting Xist (siXist). c Il-6 protein levels in the culture media of J774A.1 cells in response to LPS stimulation (1 µg /ml for 24 h) after Xist knockdown. d Fold change in the expression of IL6 mRNA in primary female monocytes from healthy human donors in response to LPS stimulation relative to control cells after XIST knockdown using siRNA (siXIST). e Relative expression of Il6 mRNA in J774A.1 cells in response to dexamethasone (Dex) treatment (10 nM 1 h prior to LPS treatment 1 µg/ml for 4 h). Il6/ IL6 and Xist expression levels normalized to Gapdh/GAPDH. f Plasma levels of IL-6 in control and CRPS patients. Female controls n = 14, female CRPS patients n = 45, male controls n = 7, male CRPS patients n = 16. For all in vitro experiments n ≥ 3. One-way ANOVA and Student’s t test were used for statistical analysis *p < 0.05, **p < 0.01, ***p < 0.001. Data represent mean ± SEM