Fig. 6.

Adoptive transfer of female splenocytes with Xist knockdown can influence inflammatory response in female mice. a Fold increase in paw thickness (relative to contralateral paw) measured 4 and 24 h after CFA administration in female mice injected with splenocytes (control or transfected with siXist). Splenocytes (10⁷ cells) were injected into the dorsal tail vein, 21 h before CFA was administered by intraplantar injection into the right hind paw (n = 4–5). b Paw withdrawal threshold to mechanical stimulation 4 h and c 24 h after CFA injection into female mice injected with splenocytes (control or transfected with siXist) 21 h before CFA injection. d Paw withdrawal latency to thermal stimulation 4 and e 24 h after CFA injection into female mice injected with splenocytes (control or transfected with siXist) 21 h before CFA injection. One-way ANOVA and Student’s t test were used for statistical analysis except for mechanical hypersensitivity in which Mann–Whitney U test was used *p < 0.05, **p < 0.01, data represent mean ± SEM (n = 4–5)