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. 2020 Sep 18;10:15347. doi: 10.1038/s41598-020-72189-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Pulse repetition frequency affects calcium signaling rates. (A) US parameter details. A slow trigger at 0.5 Hz which fired 50 times was used for both parameters. This trigger activated the two PRFs shown in blue and red which varied in pulse length and number of pulses to enable matched duty cycle. (B) Calcium signaling at two PRFs with duty cycle and power matched. Only PRF 1,500 Hz in the time bin immediately following US onset shows significant increase from baseline signaling (*p = 0.02, student’s paired t-test). Data are presented as mean ± SEM. (n.s. not significant).