Table 1.
List of identified oxidation of proteins in rat heart tissue.
| Spot no. | Protein name | Accession number | Mw/pI | Score (coverage)1 | Match fragment | Location | Function |
|---|---|---|---|---|---|---|---|
| 1 | ACON | Q9ER34 | 86.121/7.87 | 175 (37%) | 23 | Mitochondrion | Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. |
| 2 | GRP75 | P48721 | 74.097/5.97 | 189 (49%) | 24 | Mitochondrion | May play a role in the control of cell proliferation and cellular aging. |
| 3 | PCCA | P14882 | 82.198/7.59 | 113 (32%) | 16 | Mitochondrion matrix | This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. |
| 4 | ENOA | P04764 | 47.440/6.16 | 171 (54%) | 18 | Cytoplasm or cell membrane. | Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. |
| 5 | ALDH2 | P11884 | 56.966/6.63 | 125 (36%) | 14 | Mitochondrion matrix | An aldehyde + H2O + NAD+ = a carboxylate + H+ + NADH |
| 6 | KCRM | P00564 | 43.246/6.58 | 78 (26%) | 9 | Cytoplasm. | Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain, and spermatozoa. |
| 7 | ODPA | P26284 | 43.883/8.49 | 56 (20%) | 8 | Mitochondrion matrix | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle. |
| 8 | MDHM | P04636 | 36.117/8.93 | 84 (42%) | 12 | Mitochondrion matrix | (S)-malate + NAD+ = H+ + NADH + oxaloacetate |
1SwissProt 2020_02 (562253 sequences; 202348262 residues.