Figure 2.

LINC00511 is highly expressed in osteosarcoma tissues and mainly distributed in the cytoplasm, promoting cell proliferation. (a) The LINC00511 expression level in 24 paired OS tissues and the corresponding adjacent nonneoplastic tissues was examined via quantitative reverse transcription-polymerase chain reaction (qRT-PCR). GAPDH and U6 were used as internal controls. Data are represented as log₂ fold changes (cancer/normal) and defined as “<-1” for underexpression and “>1” for overexpression. In osteosarcoma tissues, the LINC00511 expression was twice as much as that in normal tissues. (b, c) The subcellular distribution of LINC00511in U2OS (b) cells and SW1353 (c) cells, with U6 and GADPH as the internal reference. (d) The efficiency of siRNA designed for LINC00511 in two osteosarcoma cell lines. (e, f) Cell proliferation was detected after transfection with siRNA targeting linc00511 or control siRNA by CCK-8 assay. Comparison of optical density (OD) values of cells with si-LINC00511 or si-NC at 450 nm at a fixed time point. ^∗P < .05, ^∗∗P < .01.