Fig. 5.

Limited function of the Ube3a paternal allele in the development of AS-like behavioral phenotypes. a–e Accelerating rotarod, nest building, open field, marble burying, and forced swim test in WT, Ube3a^m+/p−, Ube3a^m−/p+, and Ube3a^m−/p− mice (n = 15 per genotype). A repeated measures ANOVA or univariate ANOVA was used for statistical comparison of genotypes. Asterisks indicate significant effects of genotype, p < 0.001. Error bars represent mean ± SEM. f Schematic representation of several Ube3a lines used for studying the critical period, which indicates that the critical window of rescuing behavioral deficits in AS mice by gene reinstatement lies around birth and P21. Each curve depicts the level of UBE3A protein in WT, Ube3a^mLSL/Nestin-Cre, Ube3a^m+/p−, Ube3a^m−/p+, and Ube3a^m−/p− mice (this study) over time. In addition, we included the previously published result of Ube3a^mLSL mice crossed with the inducible Cag-Cre^ERT line in which gene reactivation was induced by tamoxifen injection at P21 [10]. The presence of behavioral phenotypes has been indicated for each mouse line