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. 2020 Sep 18;11:405. doi: 10.1186/s13287-020-01897-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — In vitro analysis of islet survival and function in normal conditions: a Representative confocal images, and results of b live/dead, c MTT, and d GSIS assays of tested groups (i.e., Islets, e-Islets, m-Islets, em-Islets, and emf-Islets). Confocal images and live/dead assay have been performed at days 1 and 7, and MTT and GSIS assays at day 7, in culture. Blue: live cells stained with Hoechst. Red: dead cells stained with PI. Green: AD-MSCs stained with FDA. Scale bar = 50 μm. Significant differences: b–d ^aP < 0.05: Islets vs. m-Islets or em-Islets or emf-Islets; ^bP < 0.05: m-Islets vs. em-Islets or emf-Islets; ^dP < 0.05: em-Islets vs. emf-Islets (one-way ANOVA post hoc Tukey test). b *P < 0.05: day 1 vs. day 7; d *P < 0.05: high glucose vs. low glucose (two (b, d) or one (c)-way ANOVA post hoc Tukey test)