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. 2020 Sep 18;11:405. doi: 10.1186/s13287-020-01897-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — In vivo analysis of islet survival and function (histological analysis): a representative images following H&E, insulin, and vWF immunohistochemical staining of islets in tested groups (i.e., Islets, e-Islets, m-Islets, em-Islets, and emf-Islets) that were transplanted under the kidney capsule. Black arrows indicate islets present within the representative images and red arrows indicate blood vessels within the islets; b quantification of surface area occupied by islets; c–e quantification of positive c insulin and d vWF staining. Results were analyzed with at least 15–20 islets from 5 different sections through the kidney of each animal. Significant differences: ^aP < 0.05: Islets vs. e-Islets or m-Islets or em-Islets or emf-Islets; ^bP < 0.05: e-Islets vs. m-Islets or em-Islets or emf-Islets; ^cP < 0.05: m-Islets vs. em-Islets or emf-Islets; ^dP < 0.05: em-Islets vs. emf-Islets (one-way ANOVA post hoc Tukey test)