Fig. 2.

Impact of four sequence processing approaches on the association of mode of breastfeeding with milk microbiota beta diversity. We re-processed our published 16S rRNA gene sequencing milk microbiota dataset [19] using Qiime1 and Qiime2 with or without contaminant removal resulting in four datasets (see also Fig. 1a). The Association of mode of breastfeeding with milk microbiota beta diversity was assessed on Bray-Curtis dissimilarity matrix and was tested by permutational ANOVA (PERMANOVA)