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. 2020 Aug 5;1(2):100081. doi: 10.1016/j.xpro.2020.100081

Indexing PCR mix

Reagent Final Concentration Volume per Well (μL) Volume per 384-Well Plate (μL)
Nextera PCR Master Mix (NPM) N/A 3 1,500
Nuclease-free water N/A 1 500
Total N/A 4 2,000