| Reverse Transcription Program | |||
|---|---|---|---|
| Steps | Temperature | Time | Cycles |
| RT and template-switching | 42°C | 90 min | 1 |
| Unfolding of RNA secondary structures | 50°C | 2 min | 10 |
| RT and template-switching | 42°C | 2 min | |
| Enzyme inactivation | 70°C | 15 min | 1 |
| Hold | 4°C | Forever | |
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