NSB Stock Solution
| Reagent | Final Concentration | Add to 50 mL |
|---|---|---|
| Sucrose (dry) | 166.5 mM | 2.85 g |
| MgCl2 (1 M) | 5 mM | 250 μL |
| TrisCl pH 7.4 (1 M) | 10 mM | 500 μL |
| Milli-Q water | N/A | up to 50 mL |
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