KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-HB9 | Developmental Studies Hybridoma Bank | Cat# 81.5C10; RRID:AB_2145209 |
| Rabbit polyclonal anti-TRPM8 (extracellular) | Alomone Labs | Cat# ACC-049; RRID: AB_2040254 |
| Mouse monoclonal anti-NCAM | Developmental Studies Hybridoma Bank | Cat# 4d; RRID:AB_528389 |
| Mouse monoclonal anti-HNK-1 | Sigma-Aldrich | Cat# C6680; RRID:AB_1078474 |
| Mouse monoconal anti-beta-tubulin | Developmental Studies Hybridoma Bank | Cat# E7; RRID:AB_528499 |
| Mouse monoclonal anti-neurofilament associated protein | Developmental Studies Hybridoma Bank | Cat# 3A10; RRID:AB_531874 |
| Sheep polyclonal anti-Digoxigenin-AP, Fab fragments | Roche | Cat# 11093274910; RRID:AB_514497 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Alexa Fluor 488-Dextran conjugate | Invitrogen | D22910 |
| N-(3-Aminopropyl)-2-[(3-methylphenyl)methoxy]-N-(2-thienylmethyl) benzamide hydrochloride (AMTB), TRPM8 inhibitor | Tocris | 3989 |
| (−)-menthol, TRPM8 agonist | Selleckchem.com | S4714 |
| Veratridine, voltage-gated sodium channel agonist | Sigma-Aldrich | V5754 |
| Calcicludine, voltage-gated calcium channel blocker | Alomone Labs | SPC-650 |
| ω-conotoxin GVIA, N-type calcium channel blocker | Sigma-Aldrich | C9915 |
| Flunarizine, T-type calcium channel blocker | Sigma-Aldrich | F8257 |
| Tetrodotoxin, voltage-gated sodium channel blocker | Sigma-Aldrich | T8024 |
| Tricaine Methanesulfonate (MS-222) | Western Chemical | TRS1 |
| Fluo-4, AM | Thermo Fisher Scientific | F14201 |
| Critical Commercial Assays | ||
| Apoptag-Fluorescein In Situ Apoptosis Detection Kit | Millipore | S7110 |
| Dual Luciferase Reporter Assay System | Promega | E1910 |
| MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit | Lucigen | MM070150 |
| SV Total RNA Isolation System | Promega | Z3100 |
| mMESSAGE mMACHINE T7 Transcription Kit | Ambion/ThermoFisher | AM1344 |
| mMESSAGE mMACHINE SP6 Transcription Kit | Ambion/ThermoFisher | AM1340 |
| Experimental Models: Organisms/Strains | ||
| Xenopus laevis, adult females and males | Nasco | LM00531 and LM00715 |
| Oligonucleotides | ||
| RT-PCR primer sequence Xenopus laevis trpm8: forward GAGGATGTACACTTGCTGCG; reverse TGCCTCTACTGCCATTTCCA | This paper | N/A |
| RT-PCR primer sequence Xenopus laevis odc: forward: GCCATTGTGAAGACTCTCTCCATT; reverse: ATCCGCTCGGGGGAAACTCC | This paper | N/A |
| In situ trpm8 probe: GAGCTCTAGGACTCTCTACTCAAGTTTCTCACGAAGCATCGAGTTGTCCCTGGATGAGATAGATGCTGGCAAATTCATTCAAGAAAACTTCAAGAAGAGAGAATGCGTGACATACACAAAAGATCCCAGGTCACCGGAGGGTGTCTGCAAATGTGGATATTCCCGGAGCCAGCACACTGAAGGCATACAGAGCAACCAGAATGAGAAGTGGAACTACAAGAAGAACACAAAAGATCTGCCCACAGATGCCTATGGGGATATCCAGTTTGAAACCTTTGGAAGAAGAGGAAAGTACATTCGCCTGTCCCGAGAAACTACTCCTGAAATTCTGTATGAGCTGATGACCAAATACTGGCAGCTGAAGACCCCAAACCTTATCATCTCTGTAACAGGAGGAGCCAAGAACTTCTCCCTGAAGCCACGAATGCGCAAAATCTTCAGCCGCCTCATTTACATTGCTCAGACCAAAGGAGCCTGGATATTCACAGGAGGCACTCACTATGGTCTGATGAAGTACATTGGAGAAGTGGTCAGGGATAATACTATAAGCCAAAGCTCAGAAGAAAAAGTAATTGCTATTGGTATTACAGCTTGGGGAATGATCTCCAACCGAGAATCACTGATTCGTAGCTCCAATAATGAGGGTAATTATGTTGCTCAGTATGTCATGGATGAGCAGAAGAGAGATCCACTATACTGCCTGGACAACAACCACACTCATTTGATTCTTGTAGACAATGGCACCCATGG | This paper | N/A |
| trpm8 morpholinos | Gene Tools | N/A |
| TRPM8-sbMO: 5′TGACCCTCTATGTGCCTTACTATCT3′. | ||
| TRPM8-tbMO1: 5′ATGGTCTGACAGTCATTGTAAAGCT3′. | ||
| TRPM8-tbMO2: 5′CTGCTGACACCCTTTGTATCTCTAA3′. | ||
| Control-MO: 5′CCTCTTACCTCAGTTACAATTTATA3′. | ||
| Primer sequences for detecting TRPM8 knockdown: forward: 5′-ACAAAGGGTGTCAGCAGTGT-3′; reverse: 5′-CTCCGGTGACCTGGGATCTT-3′. | This paper | N/A |
| pMO-trpm8 | [45] | N/A |
| rNav2aαβ | [5] | N/A |
| hb9-270-luc | This paper | N/A |
| hb9-270-CREB_mut-luc | This paper | N/A |
| hb9-270-AP1_mut-luc | This paper | N/A |
| Software and Algorithms | ||
| Imaris | Bitplane | Version 9.2 |
| NIS Elements | Nikon | Version 4.20 |
| Prism | Graphpad | Version 8.02 |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| Other | ||
| Bipolar temperature controller | Warner Instruments | CL-100 |
| Quick Exchange heated/cooled platform | Warner Instruments | QE-1HC |