Fig. 2.

ChiVMV HCPro directly interacts with CAT1 and CAT3 in vitro and in vivo. (A) Yeast two-hybrid assay. The ability of cells to grow on synthetic dropout medium lacking Leu, Trp, His, and Ade (-LWHA) suggested the interaction. (B) GST pull-down assay showing the interaction among HCPro, CAT1, and CAT3 in vitro. Purified CAT1–MBP, CAT2–MBP, CAT3–MBP, or MBP was incubated with HCPro–GST. After being immunoprecipitated with GST beads, the proteins were detected by protein gel blot analysis with anti-MBP or anti-GST antibodies. (C) BiFC assay. HCPro interacted with CAT1 and CAT3 in N. benthamiana leaves.Scale bars=30 μm.