Fig. 3.

Identification of HCPro domains responsible for the interaction between host factors and HCPro. (A) Schematic description of deletion mutants of ChiVMV HCPro. HCPro can be divided schematically into three regions: the N-terminus (residues 1–100), central region (residues 101–300), and C-terminus (residues 301–457). (B) Identification of the interaction specificity between CAT1 and HCPro from different sources. (C) The C-terminus of HCPro is necessary for the interaction. Different purified deletion mutants of HCPro–MBP or MBP were incubated with CAT1–GST or CAT3–GST. After being immunoprecipitated with GST beads, the proteins were detected by protein gel blot analysis with anti-MBP or anti-GST antibodies.