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. 2020 Jun 28;71(18):5656–5668. doi: 10.1093/jxb/eraa304

Fig. 8.

Fig. 8.

The local RSS activity of ChiVMV HCPro was not influenced by host factors CAT1 and CAT3. (A and D) Nicotiana benthamiana leaves were infiltrated with a mixture of three A. tumefaciens cultures carrying different constructs, as indicated in the middle panel, and photographed at 3 dpi. (B and E) Western blot analysis of protein extracts from N. benthamiana leaves infiltrated with mixtures of A. tumefaciens carrying different constructs as indicated on the left. The expression of CAT1 and CAT3 was confirmed with CAT antibody. The expression of HCPro and GFP was confirmed with HCPro and GFP antibodies, respectively. Coomassie Brilliant Blue (CBB) staining of the large subunit of Rubisco was used as a loading control. (C and F) qPCR analysis of the GFP mRNA accumulation level. Bars represent the mean and SD of values obtained from three biological repeats. Significant differences (P<0.05) are denoted by different lower case letters. (G and H) Nicotiana benthamiana leaves were infiltrated with a mixture of three A. tumefaciens cultures and photographed at 5 and 7 dpi.