Fig. 2.

Nucleotide polymorphisms and development of a functional marker in wheat TaSDIR1-4A. (A) Schematic diagram of the TaSDIR1-4A structure. The ATG start codon was designated as position 1 bp. (B) Polymorphic sites were detected in the promoter regions of TaSDIR1-4A. (C) A dCAPS marker was developed based on the –395 bp single-nucleotide polymorphism (G/A). Digestion of the amplified 200-bp fragment with Age I produced fragments of 179 bp and 21 bp for accessions with the haplotype Hap-4A-1 (A), whereas this fragment was not digested in accessions with the haplotype Hap-4A-2 (G). Marker, 100-bp DNA ladder.