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. 2020 Jun 1;71(18):5377–5388. doi: 10.1093/jxb/eraa271

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© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Phenotypic analysis of TaSDIR1-4A-silenced wheat and transgenic Arabidopsis. Virus-induced gene silencing (VIGS) of wheat was undertaken using a series of recombinant barley stripe mosaic virus (BSMV) vectors. (A) Relative expression of TaSDIR1-4A showing that transcription was lower in BSMV:TaSDIR1-4A plants than BSMV-free and BSMV:GFP plants. (B) Thousand-grain weight (TGW), (C) grain size, and (D) representative images of the grains. (E) Seed size in Arabidopsis plants in the Col-0 wild-type and T-DNA insertion mutant sdir1-1 backgrounds overexpressing (OE) TaSDIR1-4A. Vector, empty vector control. Significant differences between means were determined using one-way ANOVA followed by Tukey’s test (P<0.05).