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. 2020 May 29;71(18):5414–5424. doi: 10.1093/jxb/eraa236

Fig. 3.

Fig. 3.

Il3GAL expression induced by Dex in planta. (A) Galactanase activity in Dex::Il3GAL plants treated with Dex. Dex::Il3GAL and Dex::Il3GAL-PM plants were first grown in the absence of Dex for 2 weeks and then treated with 10 µM Dex for 2 d. The negative controls were treated with 0.1% DMSO solution for 2 d. Values indicate the total activity obtained from the soluble and wall-bound fractions. F.W., fresh weight. Data are mean values ±SD (n=3 biological replicates). Asterisks indicate significant differences between plants with and without Dex treatment (Student’s t-test, *P<0.05; **P<0.01). (B) Induction of the Il3GAL gene. Dex::Il3GAL #2 plants grown in the absence of Dex were treated with 10 µM Dex for 0.5, 1, 3, 6, 12, 24, and 48 h. The expression level of Il3GAL was determined by semi-quantitative RT–PCR. The values are shown as expression levels relative to ACTIN2. Data are mean values ±SD (n=3 biological replicates). (C) Detection of Il3GAL and Il3GAL-PM. Il3GAL and Il3GAL-PM proteins in the soluble fraction were detected by western blotting using anti-Il3GAL antibody. The arrow indicates Il3GAL or Il3GAL-PM protein.