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. 2020 May 25;71(18):5348–5364. doi: 10.1093/jxb/eraa209

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© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — RNA-sequencing analysis and qRT-PCR analysis of differentially expressed genes in roots. (A) Rates of lateral root growth for WT and OsZHD2-D plants grown under hydroponic culture conditions. (B and C) Phenotypes at 4 DAG (B) and 6 DAG (C). Scale bar=1 cm. (D) Numbers of up-regulated genes (red) and down-regulated genes (green) in OsZHD2-D roots. (E) Expression levels of ethylene biosynthesis genes. Red, genes up-regulated in OsZHD2-D; green, genes down-regulated in OsZHD2-D; gray, genes either not affected or undetected in RNA-Seq. (F–K) Expression levels of ethylene biosynthesis genes in roots at 4 DAG (F–H) and 6 DAG (I–K). Rice ubiquitin 1 (Ubi1) served as an internal control. Error bars show the SD. n=4. Statistical significance is indicated by ** (P<0.01) and *** (P<0.001).