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. 2020 Sep 18;11(9):778. doi: 10.1038/s41419-020-02972-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Molm-13, b MV4;11, and c OCI-AML3 cells were exposed to the indicated concentrations of ABT-199 ± CS055 (0.25 µM for Molm-13 and MV4;11, and 1.0 µM for OCI-AML3) or romidepsin (1.5 nM for Molm-13 and MV4;11, and 2.5–3 nM for OCI-AML3) for 48 h, after which the percentage of apoptotic cells was determined using Annexin V/PI double staining by flow cytometry. d, e Western blot analysis was performed to validate downregulation efficiency of HDAC1 and HDAC2 in MV4;11 and OCI-AML3 cells. f, g Cells were exposed to ABT-199 (50 nM for MV4;11, 1.0 µM for OCI-AML3), and then the percentage of apoptotic cells was determined by flow cytometry. Scramble shRNA-transfected cells exposed for 48 h to combined treatment with ABT-199 (50 nM for MV4;11, 1.0 µM for OCI-AML3) and CS055 (0.5 µM for MV4;11, 1.0 µM for OCI-AML3) serve as a positive control. Values indicate mean ± SD for at least three independent experiments performed in triplicate (*P < 0.05, **P < 0.01, ***P < 0.001, ns not significant).