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. 2020 Sep 18;11(9):778. doi: 10.1038/s41419-020-02972-2

Fig. 3. ABT-199 in combination with CS055 impairs colony- and tumor-forming ability of acute myeloid leukemia (AML) cells in vitro and in vivo.

Fig. 3

a AML cells were treated with ABT-199 (200 nM for MV4;11, 100 nM for Molm-13, 800 nM for OCI-AML2, 1 μM for OCI-AML3, and 4 μM for NB4) ± CS055 (0.5 μM for MV4;11/Molm-13, and 1.0 μM for the other lines) for 12 h, after which the clonogenicity assay was performed to determine the percentage of CFU (left, representative images; right, bar graphs). Values indicate mean ± SD for at least three independent experiments performed in triplicate (*P < 0.05, **P < 0.01, and ***P < 0.001; ns not significant for CS055 vs vehicle). b The scheme for the procedure of the in vivo experiment. MV4;11 cells were pre-incubated for 12 h with ABT-199 ± CS055, followed by subcutaneous injection on the left flank of nude mice. c, d Two weeks after cell inoculation, tumors were removed (c) and measured for weight and size (d).