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. 2020 Sep 18;11(9):778. doi: 10.1038/s41419-020-02972-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a OCI-AML3 (left) and MV4;11 cells (right) were exposed to the indicated concentrations of ABT-199 ± CS055 (1.0 μM for OCI-AML3; 0.5 μM for MV4;11) for 6–24 h, after which mitochondrial membrane potential (MMP) was measured using the JC-1 kit by flow cytometry. b OCI-AML3 (upper) and MV4;11 cells (lower) were pre-incubated with 20 μM Z-VAD-fmk for 2 h, followed by treatment with indicated concentrations of ABT-199 ± CS055 (1.0 μM for OCI-AML3; 0.5 μM for MV4;11) for additional 24 h. The percentage of apoptosis was then assessed by flow cytometry. c Western blot analysis was performed to monitor the expression of Mcl-1 in Molm-13, MV4;11, and OCI-AML3 cells treated with the indicated concentration of ABT-199 ± CS055. Blots were probed for β-actin or GAPDH as loading controls. d Mcl-1 was ectopically expressed in MV4;11 cells (left) or knocked down by shRNA in OCI-AML3 cells (right). e MV4;11 cells overexpressing Mcl-1 (upper) and OCI-AML3 cells with Mcl-1 knockdown (lower) were treated with indicated concentrations of ABT-199 ± CS055 for 48 h, after which the percentage of apoptosis was determined by flow cytometry. For panels a, b and e, values indicate mean ± SD for at least three independent experiments performed in triplicate (*P < 0.05, **P < 0.01, and ***P < 0.001, ns not significant).