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. 2020 Sep 18;11(9):778. doi: 10.1038/s41419-020-02972-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — MV4;11 (a, b) and OCI-AML3 cells (c, d) were treated with ABT-199 (5 nM for MV4;11, 100 nM for OCI-AML3) ± CS055 (0.5 μM for MV4;11, 1.0 μM for OCI-AML3) for 18 h, after which cells were subjected to immunofluorescent staining for Ser139 phosphorylation of histone H2A.X (H2A.X, red) and confocal microscopy (a, c; phalloidin—green, DAPI—blue; scale bars: 5 μm.) or flow cytometry for monitoring H2A.X expression (b, d). Values indicate mean ± SD for at least three independent experiments performed in triplicate (***P < 0.001, ns not significant).