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. 2020 Sep 18;9:165. doi: 10.1038/s41377-020-00401-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a PSFs obtained using a remote mirror with three steps and a sinusoidal scan motion generated by a resonant galvanometer mirror driven at 12 kHz. b Scanning over the same step mirror with a sinusoidal scan motion whose amplitude was adjusted to yield evenly illuminated laser foci. c Sinusoidal scan motion over a planar mirror inclined by 7.5°. d Same arrangement as in c, but with a threefold increased scanning amplitude and a mask to truncate the scan range optically. e Axial intensity profiles of the beams shown in c and d. XZ maximum intensity projections of transmission data are shown in a–d. Scale bar: 10 µm