Table 3.
Experimental Runs, Independent Variables and Observed Responses in 23 Factorial Design for AKBA-Loaded SNVs
| Formula | Variables | |||||
|---|---|---|---|---|---|---|
| Independent | Dependent | |||||
| X1 | X2 | X3 | Y1* | Y2* | Y3* | |
| F1 | −1 | −1 | −1 | 91.23±0.82 | 63.61±1.68 | 245.8±1.68 |
| F2 | −1 | −1 | 1 | 74.85±1.57 | 44.09±2.74 | 229.7±2.74 |
| F3 | −1 | 1 | −1 | 81.02±2.11 | 65.78±1.62 | 233.7±1.62 |
| F4 | −1 | 1 | 1 | 62.02±1.24 | 47.71±1.64 | 215.5±1.64 |
| F5 | 1 | −1 | −1 | 95.44±1.58 | 95.18±2.72 | 275.7±2.72 |
| F6 | 1 | −1 | 1 | 80.53±2.22 | 92.05±1.55 | 243.3±1.55 |
| F7# | 1 | 1 | −1 | 90.04±0.58 | 96.87±2.67 | 255.8±2.67 |
| F8 | 1 | 1 | 1 | 73.90±2.86 | 93.49±1.73 | 238.4±1.73 |
| Independent variables | Low (−1) | High (+1) | ||||
| X1: Ratio of Span60 to Tween 80 | 70:30 | 80:20 | ||||
| X2:Rotation speed (rpm) | 500 | 1000 | ||||
| X3:Sonication time (min) | 3 | 5 | ||||
Notes: Y1: EE (%), Y2: Q8h (%), Y3: PS (nm), *the values are expressed as mean ± SD; n = 3, #Optimized Formulation.
Abbreviations: EE, entrapment efficiency; Q8h, % drug released after 8h; PS, particle size.