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. Author manuscript; available in PMC: 2021 Jun 17.
Published in final edited form as: Methods Enzymol. 2020 Jun 17;641:311–328. doi: 10.1016/bs.mie.2020.04.044

Figure 2.

Figure 2.

Solid-phase synthesis of CPM3-miniPEG-KTMR. Reagents and conditions: a) Solid-phase peptide synthesis by standard Fmoc/HATU chemistry; b) 3,5-bis(bromomethyl)benzoic acid, HATU; c) 1,1’-(azanediylbis(hexane-6,1-diyl))diguanidine in 2:1 (v/v) 0.5 M NaHCO3 (pH 9)/acetonitrile; d)TFA; and e)TMR-NHS.