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Synthesis of CPM1-miniPEG-LB76TMR. Reagents and conditions: a) Solid-phase peptide synthesis using Fmoc/HATU chemistry; b) Pd(PPh3)4; c) piperidine; d) PyBOP; e) 2% (v/v) TFA in DCM; f) 3,5-bis(bromomethyl)benzoic acid, HATU; g) 1,1’-(azanediylbis(hexane-6,1-diyl))diguanidine in 2:1 (v/v) 0.5 M NaHCO3 (pH 9)/acetonitrile; h) TFA; and i) TMR-NHS.
