Table 1.

Parameters needed for dosimetric calculations with the 3DSDD model

parameter	unit	commonly used methods	comments
Nanoparticle characteristics
hydrodynamic diameter or diffusion coefficient	[nm] or [nm2 s− 1]	NTA, DLS or NTA	One of the two parameters is needed, either as average or as whole distribution.
effective density	[g cm− 3]	volumetric centrifugation method	The effective density of particles is the density of particle agglomerates that are formed for most particles in cell culture medium. For more detail please refer to [3].
Cell culture dish
dish bottom area	[cm2]	length measured or manufacturer’s information
medium filling level	[cm]	height measured or calculated
Cell system
height of cell growth at the walls	[cm]	height measured	Differentiated cell models like Caco-2 or HepaRG cells push their monolayer up the lateral wall of the culture dish during differentiation (see [5]). For undifferentiated cell models choose 0.
Cell culture medium
medium density	[g cm−3]	densitometer
medium viscosity	[mPa s]	viscometer
temperature during incubation	[°C]	thermometer
temperature during particle characterization	[°C]	thermometer
medium viscosity during particles characterization	[mPa s]	NTA
Calculation parameters
number of particles simulated	–	–	Selecting more particles yields more detailed results, but increases the computational power needed (recommendation: 10,000 particles).
simulation time	[h]	–	Corresponds to the maximal incubation time of interest.
fraction of an hour a data snapshot is taken	[h]	–	These snapshots are needed for the interactive 3D-representation of the sedimentation process. This parameter defines the time that passes between two steps in the simulation process.

Abbreviations: NTA Nanoparticle Tracing Analysis, DLS Dynamic Light Scattering