Table 3.
Subgroup analysis of cytokines to distinguish between active TB and LTBI
| IL-2 | IP-10 | IFN-γ | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| sensitivity (95% CI) | I2 (%) | specificity (95% CI) | I2 (%) | sensitivity (95% CI) | I2 (%) | specificity (95% CI) | I2 (%) | sensitivity (95% CI) | I2 (%) | specificity (95% CI) | I2 (%) | |
| Detection methods | ||||||||||||
| ELISA | 0.90 (0.68–0.99) | NA | 0.95 (0.75–1.00) | NA | 0.62 (0.54–0.70) | 90.0 | 0.72 (0.63–0.80) | 36.2 | 0.84 (0.77–0.91) | 19.2 | 0.69 (0.58–0.79) | 64.5 |
| ELISPOT | 0.85 (0.81–0.89) | 80.1 | 0.64 (0.59–0.69) | 95.9 | NA | NA | NA | NA | 0.44 (0.34–0.55) | 19.2 | 0.79 (0.70–0.87) | 72.5 |
| Luminex | 0.87 (0.77–0.93) | 67.6 | 0.53 (0.42–0.64) | 91.5 | 0.82 (0.77–0.87) | 87.3 | 0.80 (0.74–0.86) | 87.7 | NA | NA | NA | NA |
| RT-PCR | 0.96 (0.82–1.00) | NA | 0.14 (0.03–0.35) | NA | 1.00 (0.88–1.00) | NA | 0.18 (0.05–0.40) | NA | 0.71 (0.51–0.87) | NA | 0.73 (0.50–0.89) | NA |
| TB incidence | ||||||||||||
| High | 0.75 (0.66–0.83) | 0 | 0.64 (0.55–0.73) | 83.5 | 0.53 (0.41–0.65) | 87.7 | 0.69 (0.57–0.79) | 0 | 0.45 (0.36–0.55) | 0 | 0.81 (0.73–0.88) | 68.7 |
| Low | 0.90 (0.87–0.93) | 68.5 | 0.60 (0.55–0.65) | 95.4 | 0.82 (0.77–0.86) | 88.5 | 0.74 (0.68–0.79) | 90.9 | 0.86 (0.79–0.92) | 55.4 | 0.66 (0.55–0.76) | 0 |
| Stimulator of Mtb antigens | ||||||||||||
| Mtb -specific antigens | 0.84 (0.8–0.89) | 63.9 | 0.47 (0.41–0.52) | 90.4 | 0.77 (0.72–0.81) | 91.4 | 0.74 (0.68–0.79) | 89.6 | 0.70 (0.63–0.77) | 88.0 | 0.77 (0.70–0.83) | 62.2 |
| AlaDH | 0.92 (0.85–0.96) | 85.7 | 0.92 (0.87–0.96) | 91.2 | NA | NA | NA | NA | 0.48 (0.31–0.66) | NA | 0.64 (0.45–0.80) | NA |
| PPD | 0.84 (0.64–0.95) | NA | 0.58 (0.41–0.74) | NA | 0.76 (0.55–0.91) | NA | 0.67 (0.49–0.81) | NA | NA | NA | NA | NA |
Mtb-specific antigens Early secretory antigenic target-6 (ESAT-6), culture filtrate protein 10 (CFP-10), TB7.7; AlaDH L-alanine dehydrogenase, PPD Purified protein derivative, NA Not available