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. 2020 Sep 19;11(9):780. doi: 10.1038/s41419-020-02946-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Exosomes were isolated from plasma samples of 87 GC patients by stepwise centrifugation. The isolation of exosomes was confirmed by detecting CD63 and CD81 by immunoblotting (a). b Electronic microscopic image of exosomes. c Total RNAs were extracted by Trizol reagents and miRNAs level were determined by qRT-PCR. Results were analyzed by student’s t-test and P < 0.05 was considered statistically significant.