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. 2020 Sep 19;11(9):780. doi: 10.1038/s41419-020-02946-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Two HOTAIR siRNAs were transfected into GC cells separately. 48 h after transfection, total RNA was extracted and the HOTAIR level was determined by qRT-PCR. In vitro cell migration and invasion assay were processed using traditional transwell plate. b MTT assay was used to determine the relative cell viability. c The numbers of apoptotic cells in HOTAIR knocked down GC cells were counted by flow cytometry after annexin V-FITC and PI staining. Results were analyzed by One-way ANOVA and p < 0.05 was considered as significant. *P < 0.05, **P < 0.01.