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Primers used in qPCR for mRNAs
| Gene | NCBI Accession Number | Primer Sequence | Annealing Temperature (°C) | PCR Product (bp) |
|---|---|---|---|---|
| iNOS | NM_012611.3 | F:5- AACTACGTCCTATCTCCATTCTACT-3 R: 5-AACACCGCTTTCACCAAGAC-3 |
58 | 125 |
| eNOS | XM_006235872.1 | F:5- AGCCCAAACACACAGAACCT-3 R:5- AGCAGCACAAGAGTTACAAAATCC-3 |
59 | 145 |
| BDNF | XM_006234684.3 | F:5-ACGAGACCAAGTGTAATCCC-3 R:5- GCCAATTCTCTTTTTGCTATCCAT-3 |
57.5 | 137 |
| GPDH | NM_002046.7 | F:5- GGTGGGGCCAAAAGGGTCAT-3 R: 5- GGTTCACACCCATCACAAACAT-3 |
60 | 70 |
