(A) Screenshot of whole genome sequencing (WGS) data showing breakpoint junction region disrupting NIPBL. (B) Screenshot of WGS data indicating duplication of chromosome 1: 69,063,596–69,119,765 [hg19]. (C) Breakpoint junction analysis of der6_jct1, interrupting NIPBL, indicated that the rearrangement occurred de novo in the proband (left panel). Amplification of der7_jct1 (between segments 6–16 and the duplication on chromosome 1) indicated that the duplication on chromosome 1 was inherited from the father, and presumably moved en bloc with segment 6–16 during formation of the complex rearrangement (right panel).