Figure 4.

BR-Regulated Circadian Oscillation Is Compromised in bes1-ko and cca1-1lhy21 Mutants

(A and B) Bioluminescence circadian rhythms of bes1-ko (Col-0 background) (A) and cca1-1 lhy-21 (Ws-2 background) (B) mesophyll protoplasts transiently expressing the pCCA1:LUC construct in the presence or absence of 100 μM epi-BL. Mesophyll protoplasts of bes1-ko, cca1-1 lhy-21, and wild type were isolated from leaves of 3-week-old seedlings entrained MS-solid medium under ND cycles. Isolated protoplasts were transiently PEG-transfected with the pCCA1-LUC plasmid and then subjected to DD conditions to monitor bioluminescence traces. Data represent mean ± SEM (∗p < 0.05; Student's t test). Period estimates of pCCA1:LUC activity in the presence or absence of 100 μM epi-BL analyzed by FFT-NLLS in Biodare2 were shown (right panel). NS, not significant.