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Confirmation of mutations identified by targeted gene sequencing using high resolution melting assays. A, The example of missense mutation (ID3-L64F); B, The example of deletion mutation (ID3-R72_I77del); C, The example of frameshift mutation (DNMT3A-K829fs*25). T, tumor; N, normal sample; MUT, mutation; WT, wild-type; HRM, high resolution melting. All experiments were performed ≥ 3 times independently.
