Fig. 3.
Adaptation to physiological normoxia diminishes sulforaphane induced HO-1 and GCLM protein expression
bEnd 3 cells were cultured under either 18 or 5 kPa O2 for 5 d and then treated with vehicle (0.01% DMSO) or sulforaphane (SFN, 2.5 μM) for 24 h. Cell lysates were immunoblotted for HO-1 (A) and GCLM (B) expression relative to β-actin and analysed by densitometry (C–D). Data denote mean ± S.E.M., n = 3 independent bEnd.3 cultures, two-way ANOVA, *P < 0.05. **P < 0.01,***P < 0.001, ****P < 0.0001.