Table 3.
Reduced activity in assay parameters related to chemotaxis and TEM of monocytes. Effects of extracts from 3R4F, THS 2.2, and CHTP 1.2 on functional endpoints such as TEM, THP-1 cytotoxicity (7AAD), inflammation (IL-8 and TNFα release by THP-1 cells), barrier function, and THP-1 cell chemotaxis.
| THS study |
CHTP study |
|||
|---|---|---|---|---|
| 3R4F (puff/mL) | THS 2.2 (puff/mL) | 3R4F (puff/mL) | CHTP 1.2 (puff/mL) | |
| TEM IC50 | 0.049 | 0.87 | 0.067 | 0.98 |
| 7AAD IC50 | 0.085 | >3.0 | >0.5 | >4.8 |
| IL-8 peak | 0.05 | 1.0 | 0.125 | 1.52 |
| TNF peak | 0.1 | 1.0 | 0.125 | 2.03 |
| Barrier function IC50 | 0.047 | 0.79 | 0.040 | 0.68 |
| Chemotaxis IC50 | 0.016 | 0.86 | 0.055 | 0.74 |
IC50 or peak values are calculated from at least three independent experiments. TEM, transendothelial migration; 7AAD, 7-aminoactinomycin D; IL, interleukin; TNF, tumor necrosis factor; IC50, half-maximal inhibitory concentrations; THS, Tobacco Heating System; CHTP, Carbon Heated Tobacco Product.