Skip to main content
. Author manuscript; available in PMC: 2021 Jan 20.
Published in final edited form as: Nat Chem Biol. 2020 Jul 20;16(10):1052–1061. doi: 10.1038/s41589-020-0584-z

Figure 1. High-throughput screen to identify preferential IRE1/XBP1s activating compounds.

Figure 1.

A. Illustration of the screening pipeline employed to identify prioritized compounds that selectively activate the IRE1-dependent XBP1-RLuc reporter. This pipeline includes a primary screen to identify compounds that activate the XBP1-RLuc reporter, removal of compounds that activate reporters of other stress-responsive signaling pathways (e.g., the ATF6 arm of the UPR and the HSR), and structural clustering of selective activators into defined structural classes.

B. Network plot illustrating shared structural motifs among a subset of the 128 compounds identified to preferentially activate the XBP1-Rluc reporter >20%, display a maximal EC50 for reporter activation of <3 μM, and show an IC50 for toxicity of >3 μM. Prioritized compounds identified for subsequent studies are shown in red.

C. Chemical structures of our top 7 prioritized IRE1/XBP1s activators identified via high-throughput screening.