FIGURE 6.

µdot [PIN⁺] aggregates increase in size over time in act1‐122 mutants. (a) Cell lysates from cells a, d, and e were fractionated on a discontinuous (10%, 40%, 60%) sucrose gradient. Cells d and e were the same strains shown in Figure 5c,d that were grown for 60 generations. As in Figure 1, fraction 1 represents the top of the gradient, fraction 10 is the bottom. The lane labeled P shows protein found in the pellet of gradient and L is whole‐cell lysates. (b) Cell g was propagated for either 20 or 60 generations and lysates were run on discontinuous sucrose gradients. Each gel image is representative of biological replicate trials.