TABLE 1.

Cytoduction of μdot [PIN ⁺] into C10B‐H49 cells was variable

Cytoductions
Donor	Recipient	Percent cytoductants that retained [PIN +]
cell b BY4741 high [PIN +]	cell c C10B‐H49	100% (16/16)
cell d BY4741 μdot [PIN +] −	cell e C10B‐H49	95.6% (22/23)
cell d BY4741 μdot [PIN +]	cell e C10B‐H49	100% (23/23)
cell d BY4741 μdot [PIN +]	cell e C10B‐H49	3.85% (1/26)
cell d BY4741 μdot [PIN +]	cell e C10B‐H49	95.6% (22/23)

Plasmiductions
cell b BY4741 high [PIN +] + RNQ1‐GFP	cell c C10B‐H49	100% (32/32)
cell d BY4741 μdot [PIN +] + RNQ1‐GFP	cell e C10B‐H49	100% (8/8)
cell d BY4741 μdot [PIN +] + RNQ1‐GFP	cell e C10B‐H49	100% (8/8)
cell d BY4741 μdot [PIN +] + RNQ1‐GFP	cell e C10B‐H49	100% (8/8)
cell d BY4741 μdot [PIN +] + RNQ1‐GFP	cell e C10B‐H49	100% (8/8)

Cytoduction or plasmiduction were used to introduce µdot [PIN ⁺] into [pin ⁻] recipients with the C10B‐H49 genetic background. One wildtype control (M362, high [PIN ⁺] cell b) and four act1‐122 strains (M363‐M365, M378; µdot [PIN ⁺] cell d) were used as prion donors [pin ⁻] C10B‐H49 recipient cells to generate cells c and e, respectively (see Experimental Procedures). Individual cytoductants were mated to tester strains containing RNQ1‐GFP plasmids and scored for the presence of aggregates in cytoductant cell populations. The percentage of [PIN ⁺] cytoductants is shown with total number of cytoductants screened in parentheses. Since plasmiductants already contained the RNQ1‐GFP plasmid, plasmiductants were directly scored for the presence of Rnq1‐GFP aggregates in cell populations. Each line represents a single mating between donor and recipient cells.