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. Author manuscript; available in PMC: 2021 Jan 27.
Published in final edited form as: Nat Metab. 2020 Jul 27;2(9):873–881. doi: 10.1038/s42255-020-0245-2

Extended Data Fig. 1. Specificity of AMPK activation by palmitoyl-CoA and effects of co-incubations with free palmitate or coenzyme A.

Extended Data Fig. 1

a, b, Activities of AMPKα1β1γ1 (Sf9 insect cell-expressed), determined by TR-FRET SAMS assay, in the presence of coenzymes (100 μM), cofactors (100 μM) and vitamins (100 μM) (a), or following 15 min pre-incubation in the presence of palmitoyl-CoA (10 μM) ± indicated concentrations of free palmitate or coenzyme A (b). Data are shown as mean fold change in AMPK activity vs. vehicle ± s.e.m. For a, n = 3 except for folic acid, thiamine, riboflavin, pyridoxine, biotin and niacin (n = 2); for b, n = 5 (palmitate incubation) or n = 3 (coenzyme A incubation). Statistical significance was calculated using one-way ANOVA with Bonferroni’s multiple comparisons test. n represent biological independent experiments.