Extended Data Fig. 6.
Fate-mapping MacAIR during atherosclerosis progression in bone marrow transplant model Recipient Ldlr−/− mice were lethally irradiated and reconstituted with CX3CR1creER Rosa26-lsl-Tomato bone marrow. Mice were rested for 8-weeks for full hematopoietic reconstitution, then treated with tamoxifen by gavage to induce Tomato expression in CX3CR1-expressing cells, including MacAIR. (A) Three weeks after tamoxifen labeling, MacAIR remained Tomato+ (red) positive. Mice were then fed HFD and assessed for plaque development at (B) 10 days and (C) 28 days. MacAIR can be observed by Tomato-expression, whereas recruited cells were stained with CD68 (green) antibody. Samples are all en face whole mount confocal images of the aortic arch. Images are representative of n=3 for each time point.