Extended Data Fig. 2.

Mac^AIR are detected across multiple scRNA-seq approaches (A) scRNA-seq data from C57BL/6 total CD45⁺ cells was integrated with two published studies where CD45⁺ aorta cells from chow-fed Ldlr^−/− mice²² and atherosclerosis regression samples²³, identifying 10 unique clusters. (B) Top 12 enriched Mac^AIR genes were used to define Mac^AIR cluster as cluster 4, presented as relative expression. (C) Mac^AIR cells were detectable in all three datasets with a relative abundance between 2.00-9.28%. (D) cDC1 genes (Zbtb46, Flt3, Itgae, Xcr1, Snx22, Mycl, and Rab43) were interrogated against the integrated dataset, defining a unique DC population. (E) Itgae and Xcr1 gene expression plotted on integrated t-SNE cluster map.