Figure 4.

mTORC1 activity increases during the regenerative response. Protein levels of mTORC1 signaling components in duodenum as assessed by Western blotting and immunostaining. (A) Western blot analysis for p-S6 (S240/244), total S6, p-4EBP1, total 4EBP1, and loading control GAPDH. (B) p-S6 and (C) p-4EBP1 band signal was quantified and displayed as mean ± SEM (n = 3 mice/group; ∗P < .05, ∗∗P < .01 by 1-way ANOVA with Dunnett post-test). (D) Immunofluorescent images of p-S6 (green) staining with DAPI counterstain (blue). Scale bars = 100 μm.