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Rapamycin (Rap) impairs facultative stem cell contribution to intestinal regeneration. (A) Bmi1-CreER;ROSA26-LSL-lacZ mice were 12-Gy γ-irradiated, injected with tamoxifen (TX), and treated with Rap or vehicle (Veh), as shown. (B) 5 DPI duodenal sections were stained for β-galactosidase to visualize lineage traces (n = 3 mice/group). (C) Lineage tracing events were counted, and quantitative data are presented as mean ± SEM (∗P < .05 vs Veh by Student t test). Scale bars = 100 μm.
