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. 2020 Sep 11;147(17):dev186015. doi: 10.1242/dev.186015

Fig. 3.

Fig. 3.

Downregulation of HC differentiation genes and upregulation of neuronal-associated genes in the GFI1 mutant HCs. (A-F) HC-specific downregulation of Fcrlb (A) and Sema5b (B), and upregulation of the neuronal markers Gfy (C), Lhx2 (D), Neurod1 (E), POU4F1 (normally Type IC neuron-specific, F,F′) and DCX (doublecortin, normally neuron-specific, G) in the Gfi1cre/cre cochlea (n=3). Arrowheads indicate IHCs; arrows indicate OHCs; asterisks indicate nuclear POU4F1 staining. Scale bars: 20 µm. (H) Upregulation of DCX in Gfi1cre/cre vestibular HCs (n=3). Scale bar: 20 µm. (I,I′) Downregulation of OCM in the Gfi1cre/cre striolar vestibular HCs (n=3, arrows indicate loss of OCM expression). Scale bars: 50 µm. See also Figs S3 and S4.