Fig. 2.

(Continued)

(D,E) The expression levels of INSR, IRS1, total Akt and insulin-stimulated pAkt (Ser⁴⁷³/Thr³⁰⁸), and plasma membrane Glut4 content, were measured by RT-qPCR and/or WB in both organ culture tissue and isolated adipocytes from VAT samples of normal-weight subjects (n = 4 independent samples per group), under the same normoxic/hypoxic conditions as in (B,C). Representative WBs and mean densitometric analyses of WBs are shown. *P < 0.05 vs normoxia [Student's t-test].